Quantitation of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in Cell-Free Cervicovaginal Secretions: Comparison of Reverse Transcription-PCR Amplification (AMPLICOR HIV-1 MONITOR 1.5) with Enhanced-Sensitivity Branched-DNA Assay (Quantiplex 3.0)
نویسندگان
چکیده
ALI SI-MOHAMED,* LAURENT ANDREOLETTI, ISABELLE COLOMBET, MARIE-PAULE CARRENO, GLADYS LOPEZ, GILLES CHATELIER, MICHEL D. KAZATCHKINE, AND LAURENT BELEC Laboratoire de Virologie, Hôpital Européen Georges Pompidou, INSERM Unité 430 and Université Pierre et Marie Curie (Université Paris VI), Hôpital Broussais, and Département de Statistiques, Université Pierre et Marie Curie, Paris, France, and Laboratorio del Programa Nacional de Lucha contra el SIDA, Asuncion, Paraguay
منابع مشابه
Comparison of the Quantiplex version 3.0 assay and a sensitized Amplicor monitor assay for measurement of human immunodeficiency virus type 1 RNA levels in plasma samples.
This study evaluated correlation and agreement between version 3 of the Quantiplex human immunodeficiency virus type 1 (HIV-1) RNA assay (v3 branched DNA [bDNA]) and a sensitized Amplicor HIV-1 Monitor assay (reverse transcription [RT]-PCR) for the measurement of HIV RNA. Three hundred eighteen samples from 59 randomly selected, HIV-1-seropositive persons on various drug protocols from the Nati...
متن کاملEvaluation of performance of the Gen-Probe human immunodeficiency virus type 1 viral load assay using primary subtype A, C, and D isolates from Kenya.
Accurate and sensitive quantification of human immunodeficiency virus type 1 (HIV-1) RNA has been invaluable as a marker for disease prognosis and for clinical monitoring of HIV-1 disease. The first generation of commercially available HIV-1 RNA tests were optimized to detect the predominant HIV-1 subtype found in North America and Europe, subtype B. However, these tests are frequently suboptim...
متن کاملComparison of the QUANTIPLEX HIV-1 RNA 2.0 assay with the AMPLICOR HIV-1 MONITOR 1.0 assay for quantitation of levels of human immunodeficiency virus type 1 RNA in plasma of patients receiving stavudine-didanosine combination therapy.
We compared the QUANTIPLEX HIV-1 RNA 2.0 assay with the AMPLICOR HIV-1 MONITOR 1.0 assay for quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in plasma in the Stadi trail, which evaluated a stavudine plus didanosine combination therapy in 52 patients. HIV-1 RNA baseline values measured with AMPLICOR HIV-1 MONITOR 1.0 were significantly higher than those measured with QUANTIPLEX H...
متن کاملUse of active surveillance cultures in intensive care units.
3. Katsoulidou A, Petrodaskalaki M, Sypsa V, et al. Evaluation of the clinical sensitivity for the quantitation of human immunodeficiency virus type 1 RNA in plasma: comparison of the new Cobas TaqMan HIV-1 with three current HIV-RNA assays–LCx HIV RNA quantitative, Versant HIV-1 RNA 3.0 (bDNA) and Cobas Amplicor HIV-1 Monitor v1.5. J Virol Methods 2006; 131:168–74. 4. Damond F, Roquebert B, Be...
متن کاملComparative performance of three viral load assays on human immunodeficiency virus type 1 (HIV-1) isolates representing group M (subtypes A to G) and group O: LCx HIV RNA quantitative, AMPLICOR HIV-1 MONITOR version 1.5, and Quantiplex HIV-1 RNA version 3.0.
The performance of the LCx HIV RNA Quantitative (LCx HIV), AMPLICOR HIV-1 MONITOR version 1.5 (MONITOR v1.5), and Quantiplex HIV-1 RNA version 3.0 (bDNA v3.0) viral load assays was evaluated with 39 viral isolates (3 A, 7 B, 6 C, 4 D, 8 E, 4 F, 1 G, 4 mosaic, and 2 group O). Quantitation across the assay dynamic ranges was assessed using serial fivefold dilutions of the viruses. In addition, se...
متن کامل